2017-8-11 16:0:32025-05-25 21:47:48
_ec
description
DNA translocase
DNA translocase, translocation of non-segregated chromosomes prior to septum closure
locus
BSU29805
BSU_29805
geneLength
2856
2859
outlinks
bsu
BSU29805
BSU_29805
The protein
Catalyzed reaction/ biological activity
aids in moving DNA away from the closing septum, binds DNA, DNA-dependent ATPase activity [Pubmed|19818024]
the two DNA translocases [[protein|SftA]] and [[protein|SpoIIIE]] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]
aids in moving DNA away from the closing septum, binds DNA, DNA-dependent ATPase activity [Pubmed|19818024]
translocation of non-segregated chromosomes prior to septum closure
the two DNA translocases [[protein|SftA]] and [[protein|SpoIIIE]] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]
The protein
Paralogous protein(s)
[[protein|SpoIIIE]]
[[this]]
The protein
[SW|Localization]
cytoplasm (Homogeneous) [Pubmed|28110333,16479537]
small fraction at the membrane [Pubmed|28110333]
co-localizes with [[protein|FtsZ]] at nascent division sites [Pubmed|19788545]
30% of the molecules in the cytoplasm (Homogeneous) [Pubmed|29439991,28110333,16479537]
small fraction at the membrane [Pubmed|28110333]
70% of the molecules are septum-bound, co-localizes with [[protein|FtsZ]] at nascent division sites, recruitment to the septum depends on [[protein|FtsA ]][Pubmed|29439991,19788545]
Biological materials
Mutant
MGNA-A438 (ytpS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/438 NBRP B. subtilis, Japan]
1A984 ( ''sftA''::''spec''), [Pubmed|19788545], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A984&Search=1A984 BGSC]
BKE29805 (''[[gene|sftA]]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
MGNA-A438 (ytpS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/438 NBRP B. subtilis, Japan]
1A984 ( ''sftA''::''spec''), [Pubmed|19788545], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A984&Search=1A984 BGSC]
BKE29805 (''[[gene|sftA]]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
BKE29805 (Δ[[gene|sftA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29805 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTATCTCTAACTCC, downstream forward: _UP4_TAATATGGGTTCATTTTCAC
BKK29805 (Δ[[gene|sftA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29805 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTTATCTCTAACTCC, downstream forward: _UP4_TAATATGGGTTCATTTTCAC
References
Original publications
The protein
Protein family
FtsK/SpoIIIE/SftA family (with [[protein|SpoIIIE]], according to UniProt)
The protein
[SW|Domains]
[SW|FtsK domain] (aa 622-813) (according to UniProt)